MediaWaterBiotechnology applied to biological hydrogen production
The project

The BIO-H2 project proposes a system for generating biological green H2 using organic matter from wastewater as a substrate. This system is an economical and modular solution. It allows the recovery of wastewater in industry, especially if it contains a high concentration of sugars in its effluents, as is the case in the agri-food industry.

To achieve this, three lines of work are proposed: the choice of improved bacterial cultures, the optimisation of the hydrogen production process and the storage and control of hydrogen.

BIO-H2 will sensor all the critical parameters of the system, providing real-time monitoring of the entire process. A second part of this project will focus on the energy use of the hydrogen produced for self-consumption.

The objective of BIO-H2 is the biological production of green hydrogen using wastewater as input material. This green hydrogen, used as energy self-consumption in an industry, will lower the company’s energy costs and reduce its carbon footprint. In addition, the biological process will increase the biodegradability of the water, thus decreasing water treatment costs.

On the other hand, BIO-H2 will produce a waste valorisation and a complete circular economy with almost zero energy generation cost if we consider the reduction of wastewater treatment costs.

The whole system is based on the idea that it needs to operate autonomously and with minimum maintenance. Therefore, the entire hydrogen generation, water treatment and gas storage system will be digitised and sensorised in real time using 4.0 technology. All this information will be integrated into a control platform that will send automatic alerts when any of the variables go out of the established range.


This project is possible thanks to the collaboration betweenINGEOBRAS , DAB Tecnología and Rivi Grupo Técnico. It is a project coordinated by ZINNAE, the Aragonese cluster for the efficient and sustainable use of water.

Our objective is to combine knowledge in the choice of improved bacterial cultures, the optimisation of the hydrogen production process and the storage and control of hydrogen.

This synergy of technology and knowledge will result in the creation of a biological green H2 generation system, using organic matter from wastewater as a substrate.


It is expected to define an improved metabolic route, capable of increasing H2 production, and to carry out the necessary pilot tests to optimise the process by adjusting the main influencing variables. In addition, the design of the gas purification and storage system will be obtained. All of this, under the principles of industry 4.0.


This project has received aid in the form of a grant regulated by the support programme for Innovative Business Groups (AEIs) of the Ministry of Industry, Trade and Tourism. Recovery, Transformation and Resilience Plan – Funded by the European Union – NextGenerationEU..

Plan de recuperación transformación y resiliencia
financiado por la unión europea next generation
Project Information

Project acronym: BIO-H2

Full name: Biotechnology applied to biological hydrogen production

Execution period:

December 2021 to August 2022

Field of work:

Digital Technologies Projects. Industrial Research Activities

Digitalisation, LEAN WATER methodology, nano-membranes and sensorics.